By Peter Sass (eds.)
This quantity presents state of the art and novel tools on antibiotic isolation and purification, id of antimicrobial killing mechanisms, and techniques for the research and detection of microbial variation recommendations. Antibiotics: tools and Protocols courses readers via chapters on construction and layout, mode of motion, and reaction and susceptibility. Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and tips about troubleshooting and heading off recognized pitfalls.
Authoritative and state-of-the-art, Antibiotics: equipment and Protocols goals to encourage clinical paintings within the fascinating box of antibiotic research.
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Additional resources for Antibiotics: Methods and Protocols
These regulators may sense environmental signals such as nutrient starvation, oxidative stress, or the presence of competitive organisms, which can trigger the production of secondary metabolites. The computational screening of genome sequences for known DNA-binding sequences of such regulators will provide a number of candidate gene regions. The list of candidate gene regions can be minimized by excluding hits inside a comprehensive set of known biosynthetic gene clusters or hits that are associated with primary metabolism.
Another approach was based on the combination of three different similarity metrics: (a) the number of orthologous genes shared by two biosynthetic gene clusters, (b) the amount of each cluster shared in a PROmer alignment, and (c) simplified, the number of corresponding signature genes in two clusters expressed as percentage values. Creating a similarity matrix giving different weights for the different similarity indices (a: 25 %, b: 25 %, c: 50 %) allowed the implementation of these data in a distance network, clearly visualizing distinct gene cluster families .
Creating a similarity matrix giving different weights for the different similarity indices (a: 25 %, b: 25 %, c: 50 %) allowed the implementation of these data in a distance network, clearly visualizing distinct gene cluster families . The drawback of this method is the possibility that clusters producing only precursor molecules or smaller subunits can cluster in the same groups as larger and more complex secondary metabolites. 3. The third approach combined two similarity metrics: (a) the Jaccard index to measure the similarity (presence or absence) of PFAM domains from all vs.
Antibiotics: Methods and Protocols by Peter Sass (eds.)